Accessibility of DNA-epoxycellulose to sequence-specific DNA-binding proteins
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چکیده
منابع مشابه
DNA-epoxyceliulose to sequence-specific DNA-binding proteins
Phage A DNA was covalently coupled to epoxy-activated cellulose to form a stable DNA-cellulose matrix for affinity chromatography of sequence-specific DNAbinding proteins. The accessibility of three specific six-base sequences, GGATCC (BamHI), GAATTC (EcoRI) and AAGCTT (HindIII) was studied quantitatively and qualitatively by restriction analysis followed by labelling of their recessed ends. Al...
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The affinity chromatography procedure described in this unit uses DNA containing specific recognition sites for the desired protein that has been covalently linked to a solid support. Preparation of a DNA affinity resin, including cyanogen bromide (CNBr) activation of the agarose support, is described, and an alternate protocol provides a method to couple DNA to commercially available CNBr-acti...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1984
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2170331